https://sb2019.samweber.biz/story.php?title=acer-its-region-was-performed-using-10-pmoles-of-forward-and-reverse-12 Acer (ITS) region was performed using 10 pmoles of forward and reverse primers (ITS1 forward primer TCCGTAGGT GAACCTGCGG and ITS4-reverse primer TCCTCCGC TTA TTGATATGC [35], 25 L of Qiagen SYBR master mix (Qiagen, Australia) and 5 L of template in a total 50 L reaction mix. Cycling was performed using the following conditions: 95 for 15 min, 45 cycles of 95 for 30 sec, 50 for 30 sec and 72 for Tue, 24 Dec 2019 23:39:42 UTC en