https://sb2019.samweber.biz/story.php?title=from-each-fish-flash-frozen-separately-in-liquid-nitrogen-then-stored-2 From each fish, flash frozen separately in liquid nitrogen, then stored at -80 until RNA extraction. In Trial 1, blood was centrifuged (3,000 RPM, 20 minutes) and plasma collected and stored at -80 for cortisol quantification. For Trial 2 and 3, blood was centrifuged for 3 minutes (11,700 RPM, Autocrit Ultra 3, Becton Dickinson) and hematocrit measured immediately. Use of research animals compli Sat, 11 Jan 2020 18:40:38 UTC en