E difficult to be confident that reciprocal BLASTP hits between genomes are meaningful, particularly when the extent of amino acid identity falls well below levels that are typically seen for conserved housekeeping genes. For example, the general kind of anti-anti-sigma factor to which the BldG protein belongs is almost universally found among both Gram-positive firmicutes and actinobacteria; so t
Al best hit to rsbN is found next to nearly all bldN orthologues in actinomycete genomes; but, strikingly, the RsbN-like proteins are much more divergent than their BldN target or most other families of orthologous proteins of actinobacteria (Fig 6). We speculate that this may imply differences in the signal responsiveness of different RsbN proteins, thereby contributing to the differences between
Nse regulator, WhiI (Fig. 3).The key developmental regulator AdpA emerged along with complex mycelial growth and is bldA-dependent only in StreptomycineaeBldD targets also include adpA, known as bldH in S. coelicolor (den Hengst et al., 2010). AdpA has been most comprehensively described in S. griseus, in which it is the agent of the effects of the hormone-like A-factor (Horinouchi, 2002). It comp
Nse regulator, WhiI (Fig. 3).The key developmental regulator AdpA emerged along with complex mycelial growth and is bldA-dependent only in StreptomycineaeBldD targets also include adpA, known as bldH in S. coelicolor (den Hengst et al., 2010). AdpA has been most comprehensively described in S. griseus, in which it is the agent of the effects of the hormone-like A-factor (Horinouchi, 2002). It comp
Translation (Nguyen et al., 2003; Takano et al., 2003). Translational regulation is via a very rare UUA codon in the adpA mRNA, falling between the segments encoding the two domains of AdpA. UUA is the only one of the six leucine codons to comprise only A and U residues, so the corresponding TTA codon is comparatively rare in GC-rich genomes ?it occurs in only 147 chromosomal genes in S. coelicolo
Translation (Nguyen et al., 2003; Takano et al., 2003). Translational regulation is via a very rare UUA codon in the adpA mRNA, falling between the segments encoding the two domains of AdpA. UUA is the only one of the six leucine codons to comprise only A and U residues, so the corresponding TTA codon is comparatively rare in GC-rich genomes ?it occurs in only 147 chromosomal genes in S. coelicolo
Racellular functions such as protease cascades, extracellular morphogenetic peptides and secondary metabolism (Akanuma et al., 2009; Chater et al., 2010; Higo et al., 2012), but also contributing to the regulation of DnaA-mediated chromosome replication initiation (Wolanski et al., 2012). In S. griseus, many hundreds of direct targets for AdpA have been defined, and it is suspected that the unusua
Herefore be expected to overexpress rsbN, the resulting increase in anti-BldN activity might interfere with the expression of BldN-dependent genes and contribute significantly to the bald phenotype of bldD mutants. The most well-studied target of BldN is bldM, which encodes an orphan response regulator (Molle Buttner,FEMS Microbiol Rev 38 (2014) 345??2013 The Author. FEMS Microbiology Reviews pu