T direction of fold change. Primers were designed to ensure equal amplification for all species to ensure correct estimates of expression levels, as shown for collagenase-3 log2(qPCR) against log2 (microarray) shown for (B) Atlantic and (C) pink salmon. chm = chum; pnk = pink; atl = Atlantic; AK = anterior kidney; S = skin; gene acronyms are as per the primer table (Additional file 4: Table S2). A
T direction of fold change. Primers were designed to ensure equal amplification for all species to ensure correct estimates of expression levels, as shown for collagenase-3 log2(qPCR) against log2 (microarray) shown for (B) Atlantic and (C) pink salmon. chm = chum; pnk = pink; atl = Atlantic; AK = anterior kidney; S = skin; gene acronyms are as per the primer table (Additional file 4: Table S2). A
T direction of fold change. Primers were designed to ensure equal amplification for all species to ensure correct estimates of expression levels, as shown for collagenase-3 log2(qPCR) against log2 (microarray) shown for (B) Atlantic and (C) pink salmon. chm = chum; pnk = pink; atl = Atlantic; AK = anterior kidney; S = skin; gene acronyms are as per the primer table (Additional file 4: Table S2). A
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D differentially expressed genes in the skin of chum salmon at 6 days post exposure involved in immunity, proliferation, and other functions. Antiviral genes are suppressed as is seen in the anteriorSutherland et al. BMC Genomics 2014, 15:200 http://www.biomedcentral.com/1471-2164/15/Page 15 ofkidney of both Pacific salmon. Colors and formats are as described in Additional file 6: Figure S3. Addit
Ments on the manuscript and statistical discussion. Thanks to B Cox for assistance in primer testing. Thanks to anonymous reviewers for comments on the manuscript. Author details 1 Centre for Biomedical Research, Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada. 2Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency