Ments on the manuscript and statistical discussion. Thanks to B Cox for assistance in primer testing. Thanks to anonymous reviewers for comments on the manuscript. Author details 1 Centre for Biomedical Research, Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada. 2Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency
Esponse of naive Atlantic, chinook and coho salmon to experimental infection with Lepeophtheirus salmonis (Copepoda: Caligidae). Dis Aquat Organ 1992, 14(3):179?93. Krasnov A, Skugor S, Todorcevic M, Glover KA, Nilsen F: Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis, cortisol implant, and their combination. BMC Genomics 2012, 13
Esponse of naive Atlantic, chinook and coho salmon to experimental infection with Lepeophtheirus salmonis (Copepoda: Caligidae). Dis Aquat Organ 1992, 14(3):179?93. Krasnov A, Skugor S, Todorcevic M, Glover KA, Nilsen F: Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis, cortisol implant, and their combination. BMC Genomics 2012, 13
From each fish, flash frozen separately in liquid nitrogen, then stored at -80 until RNA extraction. In Trial 1, blood was centrifuged (3,000 RPM, 20 minutes) and plasma collected and stored at -80 for cortisol quantification. For Trial 2 and 3, blood was centrifuged for 3 minutes (11,700 RPM, Autocrit Ultra 3, Becton Dickinson) and hematocrit measured immediately. Use of research animals compli
Control in the anterior kidney of all three species early in the infection. Boxplot displays median and interquartile range, and circles are outliers. *denotes p < 0.05; **denotes p < 0.001. Competing interests The authors declare that they have no competing interests. Authors' contributions BJGS contributed to experimental design, performed microarray, immunoassay and qPCR work and statistical an
Esponse of naive Atlantic, chinook and coho salmon to experimental infection with Lepeophtheirus salmonis (Copepoda: Caligidae). Dis Aquat Organ 1992, 14(3):179?93. Krasnov A, Skugor S, Todorcevic M, Glover KA, Nilsen F: Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis, cortisol implant, and their combination. BMC Genomics 2012, 13
Esponse of naive Atlantic, chinook and coho salmon to experimental infection with Lepeophtheirus salmonis (Copepoda: Caligidae). Dis Aquat Organ 1992, 14(3):179?93. Krasnov A, Skugor S, Todorcevic M, Glover KA, Nilsen F: Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis, cortisol implant, and their combination. BMC Genomics 2012, 13
Ophtheirus salmonis) and in whole-body louse homogenate. J Parasitol 2000, 86(6):1199?205. Jones SRM: The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol 2001, 25(8?):841?52. Jones SRM, Fast MD, Johnson SC, Groman DB: Differential rejection of salmon lice by pink and chum salmon: disease consequences and expression of proinflammatory genes. Dis Aquat Organ