Oratory and field investigations of salmon lice Lepeophtheirus salmonis (Kr er) infestation on Atlantic salmon (Salmo salar L.) post-smolts. Aquacult Res 2000, 31(11):795?03. 51. Waite JC, Skokos D: Th17 response and inflammatory autoimmune diseases. Int J Inflamm 2012, 2012:819467. 52. Trey JE, Kushner I: The acute phase response and the hematopoietic system: the role of cytokines. Crit Rev Oncol
T direction of fold change. Primers were designed to ensure equal amplification for all species to ensure correct estimates of expression levels, as shown for collagenase-3 log2(qPCR) against log2 (microarray) shown for (B) Atlantic and (C) pink salmon. chm = chum; pnk = pink; atl = Atlantic; AK = anterior kidney; S = skin; gene acronyms are as per the primer table (Additional file 4: Table S2). A
T direction of fold change. Primers were designed to ensure equal amplification for all species to ensure correct estimates of expression levels, as shown for collagenase-3 log2(qPCR) against log2 (microarray) shown for (B) Atlantic and (C) pink salmon. chm = chum; pnk = pink; atl = Atlantic; AK = anterior kidney; S = skin; gene acronyms are as per the primer table (Additional file 4: Table S2). A
Ns in an MX3000P (Agilent) as previously described [76], with the exception of running triplicate technical replicates. Genes of interest were selected based on relevance to the study system, presence in enriched functional categories, high significance or fold change, and relevance to multiple tissues. Reference candidates were selected based on other studies, unchanging expression in infected/co
Ure S2. Multiple species utility of microarray. (A) When all species are normalized together, principal components analysis (PCA) indicates the largest variance between genus Salmo (PC1+) and Oncorhynchus (PC1-), and the second largest variance between species O. keta (PC2-) and O. gorbuscha (PC2+). The basal expression differences captured by the PCA are due to both true biological differences an
Stocks. Aquaculture 2004, 232(1?):41?2. 31. Gjerde B, Odegard J, Thorland I: Estimates of genetic variation in the susceptibility of Atlantic salmon (Salmo salar) to the salmon louse Lepeophtheirus salmonis. Aquaculture 2011, 314(1?):66?2. 32. Jones CS, Lockyer AE, Verspoor E, Secombes CJ, Noble LR: Towards selective breeding of Atlantic salmon for sea louse resistance: approaches to identify trai
Stocks. Aquaculture 2004, 232(1?):41?2. 31. Gjerde B, Odegard J, Thorland I: Estimates of genetic variation in the susceptibility of Atlantic salmon (Salmo salar) to the salmon louse Lepeophtheirus salmonis. Aquaculture 2011, 314(1?):66?2. 32. Jones CS, Lockyer AE, Verspoor E, Secombes CJ, Noble LR: Towards selective breeding of Atlantic salmon for sea louse resistance: approaches to identify trai
Ments on the manuscript and statistical discussion. Thanks to B Cox for assistance in primer testing. Thanks to anonymous reviewers for comments on the manuscript. Author details 1 Centre for Biomedical Research, Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada. 2Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency