Sing orthologues among large families of paralogues is less severe with the phylogenetically distinct ECF class of sigmas and their antisigma partners, which are more diverse than the class regulated through BldG-like cascades, and usually show high partner specificity (Staron et al., 2009). bldN, a direct target of BldD, encodes one of about 50 S. coelicolor ECF sigma factors (Bibb et al., 2000;
Tomyces spp: S. coelicolor (Sco), S. pristinaespiralis (Spr), S. griseus (Sgr) and S. venezuelae (Sven). High percentage identity is indicated by the intensity of red, and low identity by the intensity of green. A control table (`Concatenated') shows the comparisons for a concatenated set of seven universal proteins (AtpD, DnaA, DnaG, DnaK, GyrB, RecA, RpoB).2000). The distribution of convincing r
Itive search for orthologues of the 147 S. coelicolor TTA-containing genes (or in some cases gene clusters). We identified 19 that were widespread and frequently TTA-containing in 13 other Streptomyces genomes (Table 2). In addition, a further 10 genes or gene clusters frequently had TTA codons, even though their S. coelicolor orthologues were TTA-free (asterisks in Table 2). As 27/29 of the TTA-c
Tomyces spp: S. coelicolor (Sco), S. pristinaespiralis (Spr), S. griseus (Sgr) and S. venezuelae (Sven). High percentage identity is indicated by the intensity of red, and low identity by the intensity of green. A control table (`Concatenated') shows the comparisons for a concatenated set of seven universal proteins (AtpD, DnaA, DnaG, DnaK, GyrB, RecA, RpoB).2000). The distribution of convincing r
Tomyces spp: S. coelicolor (Sco), S. pristinaespiralis (Spr), S. griseus (Sgr) and S. venezuelae (Sven). High percentage identity is indicated by the intensity of red, and low identity by the intensity of green. A control table (`Concatenated') shows the comparisons for a concatenated set of seven universal proteins (AtpD, DnaA, DnaG, DnaK, GyrB, RecA, RpoB).2000). The distribution of convincing r
This general class were present in the ur-actinobacterium, giving rise to the possibility of subtle control of sigma factor activity by signals that might include morphological checkpoints (as in the case of the spoIIAA/spoIIAB genes of B. subtilis; PiggotHilbert, 2004) or stress (as in the case of sigB of B. subtilis; Price, 2000). Indeed, BldG influences the activity of the stress-responsive sig
Odon in one or another gene of the cluster.towards the start of genes that is observed in streptomycetes, and sometimes occur in conserved growthassociated genes (Chater Chandra, 2008). Interestingly, there is a strong target for BldD binding within bldA (den Hengst et al., 2010).Previously unnoticed aspects of the occurrence of conserved TTA codonsEarlier analyses had indicated that most of the
Nd their TTA codons have adaptive value to streptomycetes and not to other actinobacteria. As shown in Table 2, about half of these genes encode proteins likely to be closely implicated in gene regulation or signal transduction, although their targets are mostly unknown. They include five conserved paralogues of genes found in the whiJ cluster (see below for further discussion) and gene sets for h