Irus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation. Parasite Immunol 2007, 29(4):179?89. Fast MD, Muise DM, Easy RE, Ross NW, Johnson SC: The effects of Lepeophtheirus salmonis infections on the stress response and immunological status of Atlantic salmon (Salmo salar). Fish Shellfish Immunol 2006, 21(3):228?41. Fast MD, Ross NW, Johnson SC: Prost
Irus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation. Parasite Immunol 2007, 29(4):179?89. Fast MD, Muise DM, Easy RE, Ross NW, Johnson SC: The effects of Lepeophtheirus salmonis infections on the stress response and immunological status of Atlantic salmon (Salmo salar). Fish Shellfish Immunol 2006, 21(3):228?41. Fast MD, Ross NW, Johnson SC: Prost
Ne expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse (Lepeophtheirus salmonis) revealed bi-phasic responses coinciding with the copepod-chalimus transition. BMC Genomics 2011, 12:141.Sutherland et al. BMC Genomics 2014, 15:200 http://www.biomedcentral.com/1471-2164/15/Page 16 of25. Rolff J, Siva-Jothy MT: Invertebrate ecological immunology.
Ure S2. Multiple species utility of microarray. (A) When all species are normalized together, principal components analysis (PCA) indicates the largest variance between genus Salmo (PC1+) and Oncorhynchus (PC1-), and the second largest variance between species O. keta (PC2-) and O. gorbuscha (PC2+). The basal expression differences captured by the PCA are due to both true biological differences an
Ure S2. Multiple species utility of microarray. (A) When all species are normalized together, principal components analysis (PCA) indicates the largest variance between genus Salmo (PC1+) and Oncorhynchus (PC1-), and the second largest variance between species O. keta (PC2-) and O. gorbuscha (PC2+). The basal expression differences captured by the PCA are due to both true biological differences an
Ure S2. Multiple species utility of microarray. (A) When all species are normalized together, principal components analysis (PCA) indicates the largest variance between genus Salmo (PC1+) and Oncorhynchus (PC1-), and the second largest variance between species O. keta (PC2-) and O. gorbuscha (PC2+). The basal expression differences captured by the PCA are due to both true biological differences an
Cal replicates were found to be within 0.5 Ct for all samples. The interplate calibrator used to compare across plates within a gene had a
N set of the Venn diagram). During differential expression testing, each species was therefore normalized separately, and indirectly compared. Data shown: anterior kidney. Additional file 4: Table S2. Primers for qPCR. Additional file 5: Table S3. Differentially expressed gene lists. Additional file 6: Figure S3. Differentially expressed cellular stress, prostaglandin, coagulation and other relate