The analysis mixture composed of 1.5?mL regarding 0.1?mol?L-1 phosphate barrier(PB, pH=7.A few), 3.3?mL of a single.3?mol?L-1 Methionine, 0.3?mol of 750 umol L-1 NBT, 0.3?mL of Hundred umol L-1 EDTA-Na2, 2.3?mL involving Riboflavin as well as 3.25?mL regarding distilled water. A total volume of Three or more.0?mL associated with assay blend had been reached by having Zero.05?mL of enzyme draw out