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Four, One hundred fifty millimeters NaCl, A couple of millimeters EDTA, 0.5% Nonidet P-40) in addition protease inhibitors (1 ��g/ml aprotinin, One particular ��g/ml leupeptin, A single ��g/ml pepstatin Any). Your lysates have been accumulated, centrifuged in 13,Thousand revolutions per minute with regard to 15 minute, and quantified for overall healthy proteins by way of a bicinchoninic acidity p