Llection and maintenance, exposures and sample collection, and hematocrit analysis. BFK and SRMJ conceived of the study, designed the experiment and assisted in analyses. All authors have read and approve of the manuscript. Acknowledgements This research was funded by Genome BC, the Province of British Columbia, the Department of Fisheries and Oceans Canada (DFO), NSERC, the University of Victoria
Llection and maintenance, exposures and sample collection, and hematocrit analysis. BFK and SRMJ conceived of the study, designed the experiment and assisted in analyses. All authors have read and approve of the manuscript. Acknowledgements This research was funded by Genome BC, the Province of British Columbia, the Department of Fisheries and Oceans Canada (DFO), NSERC, the University of Victoria
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T direction of fold change. Primers were designed to ensure equal amplification for all species to ensure correct estimates of expression levels, as shown for collagenase-3 log2(qPCR) against log2 (microarray) shown for (B) Atlantic and (C) pink salmon. chm = chum; pnk = pink; atl = Atlantic; AK = anterior kidney; S = skin; gene acronyms are as per the primer table (Additional file 4: Table S2). A
T direction of fold change. Primers were designed to ensure equal amplification for all species to ensure correct estimates of expression levels, as shown for collagenase-3 log2(qPCR) against log2 (microarray) shown for (B) Atlantic and (C) pink salmon. chm = chum; pnk = pink; atl = Atlantic; AK = anterior kidney; S = skin; gene acronyms are as per the primer table (Additional file 4: Table S2). A
Ne expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse (Lepeophtheirus salmonis) revealed bi-phasic responses coinciding with the copepod-chalimus transition. BMC Genomics 2011, 12:141.Sutherland et al. BMC Genomics 2014, 15:200 http://www.biomedcentral.com/1471-2164/15/Page 16 of25. Rolff J, Siva-Jothy MT: Invertebrate ecological immunology.