Eir infant's second birthday. Instructions on sample collection were provided at the initial visit by research staff who also demonstrated the technique by undertaking the initial nasal swab specimen shortly after delivery of the newborn baby. In addition, parents were given written instructions on how to collect nasal swab specimens. They also received regular text messages, emails or telephone c
He first year mould was seen in some samples as they arrived in the laboratory and we became concerned about the impact of this contaminant upon sample integrity. Therefore, as part of the ORChID study, we undertook a broader investigation of sample quality, examining collection and transportation, and how these impact on respiratory virus detection. Our objectives were first to determine the qual
Standard deviation) time from collection until being frozen in the laboratory for samples with no observed mould was 2.9 (3.0) days. In comparison for low level mould it was 4.9 (3.6) days (crude mean difference compared with no mould; 95 confidence interval (CI) = 1.7; 1.4 ?2.1 days), for medium level mould it was 7.4 (4.9) days (3.9; 3.4 ?4.3), and for high level mould 11.4 (10.7) days (7.1; 6.
Standard deviation) time from collection until being frozen in the laboratory for samples with no observed mould was 2.9 (3.0) days. In comparison for low level mould it was 4.9 (3.6) days (crude mean difference compared with no mould; 95 confidence interval (CI) = 1.7; 1.4 ?2.1 days), for medium level mould it was 7.4 (4.9) days (3.9; 3.4 ?4.3), and for high level mould 11.4 (10.7) days (7.1; 6.
Control testing were screened for respiratory viruses using previously optimized and described PCR and reverse transcriptase PCR assays. Virus testing assays included: rhinovirus (RV) [26], influenza viruses (A and B) [27], respiratory syncytial viruses (A and B) [28], parainfluenza viruses (1?) [29], human adenoviruses [22], humanTable 1 Oligonucleotide primers for equine herpes virus-1 (EHV 1) a
Acer (ITS) region was performed using 10 pmoles of forward and reverse primers (ITS1 forward primer TCCGTAGGT GAACCTGCGG and ITS4-reverse primer TCCTCCGC TTA TTGATATGC [35], 25 L of Qiagen SYBR master mix (Qiagen, Australia) and 5 L of template in a total 50 L reaction mix. Cycling was performed using the following conditions: 95 for 15 min, 45 cycles of 95 for 30 sec, 50 for 30 sec and 72 for
Acer (ITS) region was performed using 10 pmoles of forward and reverse primers (ITS1 forward primer TCCGTAGGT GAACCTGCGG and ITS4-reverse primer TCCTCCGC TTA TTGATATGC [35], 25 L of Qiagen SYBR master mix (Qiagen, Australia) and 5 L of template in a total 50 L reaction mix. Cycling was performed using the following conditions: 95 for 15 min, 45 cycles of 95 for 30 sec, 50 for 30 sec and 72 for
T ambient temperature has limited or no impact on respiratory virus detection by PCR [14,20,21], although investigating further the effects of transporting samples for extended periods and at higher temperatures was highlighted in one study [20]. The observational research in childhood infectious diseases (ORChID) project is a longitudinal, communitybased, dynamic birth cohort study, which seeks t