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Rulequart46

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T the direct or indirect targets of WhiJ regulation or about the role of the antisigma-like protein (SCO4544). The apparently repressing action of the whiJ locus raises the possibility that some or all of its paralogues may also act as developmental brakes. If so, it may be that during `normal' colony development on laboratory media, these brakes are all off ?in other words, all relevant checkpoin
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Tively long-established clusters. Twelve other S. coelicolor whiJ-like genes were represented in around half of streptomycetes (SCO2381*, 2865, 2869*, 3365*, 4176, 4301, 4678, 4998, 6129, 6537, 6629*, 7579) [asterisks indicate occurrence also in some other complex actinomycete(s)]; while seven others were found in four or fewer streptomycetes (SCO0704, 2246, 2253, 4543, 5125,Fig. 7. Phylogenetic a
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N each cluster co-evolved (results not shown). WhiJ paralogues in S. coelicolor vary considerably in their conservation in other organisms. One (SCO3421) was present in nearly all complex actinomycetes, and the adjacent gene encoding a likely antisigma factor nearly always contained a TTA codon in Streptomycineae (but in no other groups). Another present in all Streptomycineae (SCO4441) was also w
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N each cluster co-evolved (results not shown). WhiJ paralogues in S. coelicolor vary considerably in their conservation in other organisms. One (SCO3421) was present in nearly all complex actinomycetes, and the adjacent gene encoding a likely antisigma factor nearly always contained a TTA codon in Streptomycineae (but in no other groups). Another present in all Streptomycineae (SCO4441) was also w
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Factor (AbaA-like) Unknown Unknown SapB biosynthetic enzyme; regulator of SapB biosynthesis Possible phosphotransferase Component of conservon6/13; 7/14 9/11 6/10 8/13 8/12 9/14 8/14 3/11; 5/13 14/14 5/14 12/14 6/14 7/13 5/14 9/14 8/11 12/14 6/14 6/14 7/14 6/13 5/14 5/13 7/14 2/12; 7/12 10/12 9/(? (?(TTA) (? (TTA) (TTA) (??40040 (? 43220 (? ??49090 50850 (TTA) 51100 (TTA) 26520 19600 (? ???16190 3
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Factor (AbaA-like) Unknown Unknown SapB biosynthetic enzyme; regulator of SapB biosynthesis Possible phosphotransferase Component of conservon6/13; 7/14 9/11 6/10 8/13 8/12 9/14 8/14 3/11; 5/13 14/14 5/14 12/14 6/14 7/13 5/14 9/14 8/11 12/14 6/14 6/14 7/14 6/13 5/14 5/13 7/14 2/12; 7/12 10/12 9/(? (?(TTA) (? (TTA) (TTA) (??40040 (? 43220 (? ??49090 50850 (TTA) 51100 (TTA) 26520 19600 (? ???16190 3
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Can hydrolase activity (Mukamolova et al., 2006; Haiser et al., 2009). Mutational analysis indicates considerable functional redundancy among RPFs in mycobacteria (reviewed by Kana Mizrahi, 2010), but phenotypic effects were observed in S. coelicolor when SCO3097 was disrupted: the mutant produced thin-walled, heat-sensitive, irregular spores in chains that tended not to separate as readily as w
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Can hydrolase activity (Mukamolova et al., 2006; Haiser et al., 2009). Mutational analysis indicates considerable functional redundancy among RPFs in mycobacteria (reviewed by Kana Mizrahi, 2010), but phenotypic effects were observed in S. coelicolor when SCO3097 was disrupted: the mutant produced thin-walled, heat-sensitive, irregular spores in chains that tended not to separate as readily as w