Esponse of naive Atlantic, chinook and coho salmon to experimental infection with Lepeophtheirus salmonis (Copepoda: Caligidae). Dis Aquat Organ 1992, 14(3):179?93. Krasnov A, Skugor S, Todorcevic M, Glover KA, Nilsen F: Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis, cortisol implant, and their combination. BMC Genomics 2012, 13
Esponse of naive Atlantic, chinook and coho salmon to experimental infection with Lepeophtheirus salmonis (Copepoda: Caligidae). Dis Aquat Organ 1992, 14(3):179?93. Krasnov A, Skugor S, Todorcevic M, Glover KA, Nilsen F: Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis, cortisol implant, and their combination. BMC Genomics 2012, 13
Llection and maintenance, exposures and sample collection, and hematocrit analysis. BFK and SRMJ conceived of the study, designed the experiment and assisted in analyses. All authors have read and approve of the manuscript. Acknowledgements This research was funded by Genome BC, the Province of British Columbia, the Department of Fisheries and Oceans Canada (DFO), NSERC, the University of Victoria
Llection and maintenance, exposures and sample collection, and hematocrit analysis. BFK and SRMJ conceived of the study, designed the experiment and assisted in analyses. All authors have read and approve of the manuscript. Acknowledgements This research was funded by Genome BC, the Province of British Columbia, the Department of Fisheries and Oceans Canada (DFO), NSERC, the University of Victoria
N set of the Venn diagram). During differential expression testing, each species was therefore normalized separately, and indirectly compared. Data shown: anterior kidney. Additional file 4: Table S2. Primers for qPCR. Additional file 5: Table S3. Differentially expressed gene lists. Additional file 6: Figure S3. Differentially expressed cellular stress, prostaglandin, coagulation and other relate
Cal replicates were found to be within 0.5 Ct for all samples. The interplate calibrator used to compare across plates within a gene had a
Ure S2. Multiple species utility of microarray. (A) When all species are normalized together, principal components analysis (PCA) indicates the largest variance between genus Salmo (PC1+) and Oncorhynchus (PC1-), and the second largest variance between species O. keta (PC2-) and O. gorbuscha (PC2+). The basal expression differences captured by the PCA are due to both true biological differences an
Ure S2. Multiple species utility of microarray. (A) When all species are normalized together, principal components analysis (PCA) indicates the largest variance between genus Salmo (PC1+) and Oncorhynchus (PC1-), and the second largest variance between species O. keta (PC2-) and O. gorbuscha (PC2+). The basal expression differences captured by the PCA are due to both true biological differences an