Ribed above except that only one tissue was tested for efficiency, and standard curves were approximately in the range of the sample values. All technical replicates were within 0.5 Ct for 224/228 combinations. Primer thermal regimes for these genes were reported previously [33].Data accessibilityGene expression data files have been uploaded to Gene Expression Omnibus (GSE48337).Additional filesAd
Irus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation. Parasite Immunol 2007, 29(4):179?89. Fast MD, Muise DM, Easy RE, Ross NW, Johnson SC: The effects of Lepeophtheirus salmonis infections on the stress response and immunological status of Atlantic salmon (Salmo salar). Fish Shellfish Immunol 2006, 21(3):228?41. Fast MD, Ross NW, Johnson SC: Prost
Ophtheirus salmonis) and in whole-body louse homogenate. J Parasitol 2000, 86(6):1199?205. Jones SRM: The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol 2001, 25(8?):841?52. Jones SRM, Fast MD, Johnson SC, Groman DB: Differential rejection of salmon lice by pink and chum salmon: disease consequences and expression of proinflammatory genes. Dis Aquat Organ
Ophtheirus salmonis) and in whole-body louse homogenate. J Parasitol 2000, 86(6):1199?205. Jones SRM: The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol 2001, 25(8?):841?52. Jones SRM, Fast MD, Johnson SC, Groman DB: Differential rejection of salmon lice by pink and chum salmon: disease consequences and expression of proinflammatory genes. Dis Aquat Organ
N set of the Venn diagram). During differential expression testing, each species was therefore normalized separately, and indirectly compared. Data shown: anterior kidney. Additional file 4: Table S2. Primers for qPCR. Additional file 5: Table S3. Differentially expressed gene lists. Additional file 6: Figure S3. Differentially expressed cellular stress, prostaglandin, coagulation and other relate
N set of the Venn diagram). During differential expression testing, each species was therefore normalized separately, and indirectly compared. Data shown: anterior kidney. Additional file 4: Table S2. Primers for qPCR. Additional file 5: Table S3. Differentially expressed gene lists. Additional file 6: Figure S3. Differentially expressed cellular stress, prostaglandin, coagulation and other relate
Ments on the manuscript and statistical discussion. Thanks to B Cox for assistance in primer testing. Thanks to anonymous reviewers for comments on the manuscript. Author details 1 Centre for Biomedical Research, Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada. 2Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency
A lice on juvenile Pacific salmon in the Gulf Islands area of British Columbia, Canada. Aquaculture 2009, 297(1?):31?7. Wagner GN, Fast MD, Johnson SC: Physiology and immunology of Lepeophtheirus salmonis infections of salmonids. Trends Parasitol 2008, 24(4):176?83. Jones SRM, Kim E, Bennett W: Early development of resistance to the salmon louse, Lepeophtheirus salmonis (Kr er), in juvenile pink s