Irus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation. Parasite Immunol 2007, 29(4):179?89. Fast MD, Muise DM, Easy RE, Ross NW, Johnson SC: The effects of Lepeophtheirus salmonis infections on the stress response and immunological status of Atlantic salmon (Salmo salar). Fish Shellfish Immunol 2006, 21(3):228?41. Fast MD, Ross NW, Johnson SC: Prost
D differentially expressed genes in the skin of chum salmon at 6 days post exposure involved in immunity, proliferation, and other functions. Antiviral genes are suppressed as is seen in the anteriorSutherland et al. BMC Genomics 2014, 15:200 http://www.biomedcentral.com/1471-2164/15/Page 15 ofkidney of both Pacific salmon. Colors and formats are as described in Additional file 6: Figure S3. Addit
Llection and maintenance, exposures and sample collection, and hematocrit analysis. BFK and SRMJ conceived of the study, designed the experiment and assisted in analyses. All authors have read and approve of the manuscript. Acknowledgements This research was funded by Genome BC, the Province of British Columbia, the Department of Fisheries and Oceans Canada (DFO), NSERC, the University of Victoria
Ure S2. Multiple species utility of microarray. (A) When all species are normalized together, principal components analysis (PCA) indicates the largest variance between genus Salmo (PC1+) and Oncorhynchus (PC1-), and the second largest variance between species O. keta (PC2-) and O. gorbuscha (PC2+). The basal expression differences captured by the PCA are due to both true biological differences an
Ure S2. Multiple species utility of microarray. (A) When all species are normalized together, principal components analysis (PCA) indicates the largest variance between genus Salmo (PC1+) and Oncorhynchus (PC1-), and the second largest variance between species O. keta (PC2-) and O. gorbuscha (PC2+). The basal expression differences captured by the PCA are due to both true biological differences an
Ophtheirus salmonis) and in whole-body louse homogenate. J Parasitol 2000, 86(6):1199?205. Jones SRM: The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol 2001, 25(8?):841?52. Jones SRM, Fast MD, Johnson SC, Groman DB: Differential rejection of salmon lice by pink and chum salmon: disease consequences and expression of proinflammatory genes. Dis Aquat Organ
D differentially expressed genes in the skin of chum salmon at 6 days post exposure involved in immunity, proliferation, and other functions. Antiviral genes are suppressed as is seen in the anteriorSutherland et al. BMC Genomics 2014, 15:200 http://www.biomedcentral.com/1471-2164/15/Page 15 ofkidney of both Pacific salmon. Colors and formats are as described in Additional file 6: Figure S3. Addit
Ns in an MX3000P (Agilent) as previously described [76], with the exception of running triplicate technical replicates. Genes of interest were selected based on relevance to the study system, presence in enriched functional categories, high significance or fold change, and relevance to multiple tissues. Reference candidates were selected based on other studies, unchanging expression in infected/co