Ribed above except that only one tissue was tested for efficiency, and standard curves were approximately in the range of the sample values. All technical replicates were within 0.5 Ct for 224/228 combinations. Primer thermal regimes for these genes were reported previously [33].Data accessibilityGene expression data files have been uploaded to Gene Expression Omnibus (GSE48337).Additional filesAd
Ophtheirus salmonis) and in whole-body louse homogenate. J Parasitol 2000, 86(6):1199?205. Jones SRM: The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol 2001, 25(8?):841?52. Jones SRM, Fast MD, Johnson SC, Groman DB: Differential rejection of salmon lice by pink and chum salmon: disease consequences and expression of proinflammatory genes. Dis Aquat Organ
Ophtheirus salmonis) and in whole-body louse homogenate. J Parasitol 2000, 86(6):1199?205. Jones SRM: The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol 2001, 25(8?):841?52. Jones SRM, Fast MD, Johnson SC, Groman DB: Differential rejection of salmon lice by pink and chum salmon: disease consequences and expression of proinflammatory genes. Dis Aquat Organ
Ments on the manuscript and statistical discussion. Thanks to B Cox for assistance in primer testing. Thanks to anonymous reviewers for comments on the manuscript. Author details 1 Centre for Biomedical Research, Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada. 2Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency
T direction of fold change. Primers were designed to ensure equal amplification for all species to ensure correct estimates of expression levels, as shown for collagenase-3 log2(qPCR) against log2 (microarray) shown for (B) Atlantic and (C) pink salmon. chm = chum; pnk = pink; atl = Atlantic; AK = anterior kidney; S = skin; gene acronyms are as per the primer table (Additional file 4: Table S2). A
Llection and maintenance, exposures and sample collection, and hematocrit analysis. BFK and SRMJ conceived of the study, designed the experiment and assisted in analyses. All authors have read and approve of the manuscript. Acknowledgements This research was funded by Genome BC, the Province of British Columbia, the Department of Fisheries and Oceans Canada (DFO), NSERC, the University of Victoria
Llection and maintenance, exposures and sample collection, and hematocrit analysis. BFK and SRMJ conceived of the study, designed the experiment and assisted in analyses. All authors have read and approve of the manuscript. Acknowledgements This research was funded by Genome BC, the Province of British Columbia, the Department of Fisheries and Oceans Canada (DFO), NSERC, the University of Victoria
Esponse of naive Atlantic, chinook and coho salmon to experimental infection with Lepeophtheirus salmonis (Copepoda: Caligidae). Dis Aquat Organ 1992, 14(3):179?93. Krasnov A, Skugor S, Todorcevic M, Glover KA, Nilsen F: Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis, cortisol implant, and their combination. BMC Genomics 2012, 13