Er or others), season specimen collected, and time from specimen collection to being frozen in the laboratory. Analyses were conducted using Stata statistical software v.11.0 (StataCorp, College Station, TX, USA).however 10.9 of swabs were received more than 7-days after their collection.Excluded samples:For EHV1 extraction and inhibition testing, 42 (0.81 ) DNA extracts failed the EHV1 criteria.
Rtile range 2?) days;Of the remaining 3366 samples, there were 2718 (80.7 ) samples positive for ERV3 with PCR amplification Ct values ranging from 23?5 (median 36) cycles. Overall, ERV3 was not detected in 649 (19.2 ) samples. During the first 8-months of batching and screening conducted in the laboratory, the number of ERV3 negative samples ranged from 11 to 25 in each 92 extraction run with a m
Rtile range 2?) days;Of the remaining 3366 samples, there were 2718 (80.7 ) samples positive for ERV3 with PCR amplification Ct values ranging from 23?5 (median 36) cycles. Overall, ERV3 was not detected in 649 (19.2 ) samples. During the first 8-months of batching and screening conducted in the laboratory, the number of ERV3 negative samples ranged from 11 to 25 in each 92 extraction run with a m
Er or others), season specimen collected, and time from specimen collection to being frozen in the laboratory. Analyses were conducted using Stata statistical software v.11.0 (StataCorp, College Station, TX, USA).however 10.9 of swabs were received more than 7-days after their collection.Excluded samples:For EHV1 extraction and inhibition testing, 42 (0.81 ) DNA extracts failed the EHV1 criteria.
Erved on 28.2 of swabs. In comparison mould detection rates were 31.0 in spring (crude odds ratio (OR); 95 CI = 1.08; 0.87 ?1.34), 15.8 in autumn (0.47; 0.37 ?0.59) and 13.7 in winter (0.40; 0.29 ?0.53). When considering samples that contained mould, thereTable 2 Results for respiratory viruses screening from 3366 parent collected nasal swab specimens between July 2011 and July 2012 and fulfi
Erved on 28.2 of swabs. In comparison mould detection rates were 31.0 in spring (crude odds ratio (OR); 95 CI = 1.08; 0.87 ?1.34), 15.8 in autumn (0.47; 0.37 ?0.59) and 13.7 in winter (0.40; 0.29 ?0.53). When considering samples that contained mould, thereTable 2 Results for respiratory viruses screening from 3366 parent collected nasal swab specimens between July 2011 and July 2012 and fulfi
Mould was observed growing on a small number of nasal swabs at the time of their arrival at the Laboratory. In light of this observation, before extraction all swabs were inspected visually for mould and were assigned a semi-qualitative score according to a sliding scale (0 to 3), whereby 0 = no mould observed, 1 = low, 2 = medium, and 3 = high levels of visible mould present. DNA sequencing was u
Acer (ITS) region was performed using 10 pmoles of forward and reverse primers (ITS1 forward primer TCCGTAGGT GAACCTGCGG and ITS4-reverse primer TCCTCCGC TTA TTGATATGC [35], 25 L of Qiagen SYBR master mix (Qiagen, Australia) and 5 L of template in a total 50 L reaction mix. Cycling was performed using the following conditions: 95 for 15 min, 45 cycles of 95 for 30 sec, 50 for 30 sec and 72 for