Ments on the manuscript and statistical discussion. Thanks to B Cox for assistance in primer testing. Thanks to anonymous reviewers for comments on the manuscript. Author details 1 Centre for Biomedical Research, Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada. 2Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency
Ments on the manuscript and statistical discussion. Thanks to B Cox for assistance in primer testing. Thanks to anonymous reviewers for comments on the manuscript. Author details 1 Centre for Biomedical Research, Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada. 2Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency
A lice on juvenile Pacific salmon in the Gulf Islands area of British Columbia, Canada. Aquaculture 2009, 297(1?):31?7. Wagner GN, Fast MD, Johnson SC: Physiology and immunology of Lepeophtheirus salmonis infections of salmonids. Trends Parasitol 2008, 24(4):176?83. Jones SRM, Kim E, Bennett W: Early development of resistance to the salmon louse, Lepeophtheirus salmonis (Kr er), in juvenile pink s
Ribed above except that only one tissue was tested for efficiency, and standard curves were approximately in the range of the sample values. All technical replicates were within 0.5 Ct for 224/228 combinations. Primer thermal regimes for these genes were reported previously [33].Data accessibilityGene expression data files have been uploaded to Gene Expression Omnibus (GSE48337).Additional filesAd
Irus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation. Parasite Immunol 2007, 29(4):179?89. Fast MD, Muise DM, Easy RE, Ross NW, Johnson SC: The effects of Lepeophtheirus salmonis infections on the stress response and immunological status of Atlantic salmon (Salmo salar). Fish Shellfish Immunol 2006, 21(3):228?41. Fast MD, Ross NW, Johnson SC: Prost
Instructions with the oncolumn DNase I digestion. The RNA was quality checked by agarose gel electrophoresis and quantified by spectrometry (NanoDrop-1000).Cortisol, weight and hematocrit analysesCortisol levels in plasma were tested by immunoassay of 20 l samples (ParameterTM, R D Systems). Samples were run in duplicate, and a standard curve and interplate calibrator sample was run on each plate.
Usly described [10]. Data analysis was performed using qbasePLUS (Biogazelle) and reference gene stability was tested using geNorm [81]. The three most stable reference genes chosen for the current analysis in all species and all tissues were dynein light chain 1 cytoplasmic, U6 snRNA-associated Sm-like protein lsm8, and mRNA turnover protein 4 homolog with collective M (and CV) values for Atlanti
A lice on juvenile Pacific salmon in the Gulf Islands area of British Columbia, Canada. Aquaculture 2009, 297(1?):31?7. Wagner GN, Fast MD, Johnson SC: Physiology and immunology of Lepeophtheirus salmonis infections of salmonids. Trends Parasitol 2008, 24(4):176?83. Jones SRM, Kim E, Bennett W: Early development of resistance to the salmon louse, Lepeophtheirus salmonis (Kr er), in juvenile pink s