Ophtheirus salmonis) and in whole-body louse homogenate. J Parasitol 2000, 86(6):1199?205. Jones SRM: The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol 2001, 25(8?):841?52. Jones SRM, Fast MD, Johnson SC, Groman DB: Differential rejection of salmon lice by pink and chum salmon: disease consequences and expression of proinflammatory genes. Dis Aquat Organ
T direction of fold change. Primers were designed to ensure equal amplification for all species to ensure correct estimates of expression levels, as shown for collagenase-3 log2(qPCR) against log2 (microarray) shown for (B) Atlantic and (C) pink salmon. chm = chum; pnk = pink; atl = Atlantic; AK = anterior kidney; S = skin; gene acronyms are as per the primer table (Additional file 4: Table S2). A
T direction of fold change. Primers were designed to ensure equal amplification for all species to ensure correct estimates of expression levels, as shown for collagenase-3 log2(qPCR) against log2 (microarray) shown for (B) Atlantic and (C) pink salmon. chm = chum; pnk = pink; atl = Atlantic; AK = anterior kidney; S = skin; gene acronyms are as per the primer table (Additional file 4: Table S2). A
Llection and maintenance, exposures and sample collection, and hematocrit analysis. BFK and SRMJ conceived of the study, designed the experiment and assisted in analyses. All authors have read and approve of the manuscript. Acknowledgements This research was funded by Genome BC, the Province of British Columbia, the Department of Fisheries and Oceans Canada (DFO), NSERC, the University of Victoria
D differentially expressed genes in the skin of chum salmon at 6 days post exposure involved in immunity, proliferation, and other functions. Antiviral genes are suppressed as is seen in the anteriorSutherland et al. BMC Genomics 2014, 15:200 http://www.biomedcentral.com/1471-2164/15/Page 15 ofkidney of both Pacific salmon. Colors and formats are as described in Additional file 6: Figure S3. Addit
Ophtheirus salmonis) and in whole-body louse homogenate. J Parasitol 2000, 86(6):1199?205. Jones SRM: The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol 2001, 25(8?):841?52. Jones SRM, Fast MD, Johnson SC, Groman DB: Differential rejection of salmon lice by pink and chum salmon: disease consequences and expression of proinflammatory genes. Dis Aquat Organ
Ophtheirus salmonis) and in whole-body louse homogenate. J Parasitol 2000, 86(6):1199?205. Jones SRM: The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol 2001, 25(8?):841?52. Jones SRM, Fast MD, Johnson SC, Groman DB: Differential rejection of salmon lice by pink and chum salmon: disease consequences and expression of proinflammatory genes. Dis Aquat Organ
Irus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation. Parasite Immunol 2007, 29(4):179?89. Fast MD, Muise DM, Easy RE, Ross NW, Johnson SC: The effects of Lepeophtheirus salmonis infections on the stress response and immunological status of Atlantic salmon (Salmo salar). Fish Shellfish Immunol 2006, 21(3):228?41. Fast MD, Ross NW, Johnson SC: Prost