Reference pool). Experimental samples (labelled with Cy5) included 9?1 biological replicates for the infected individuals and 9?0 biological replicates for time-matched controls. Anterior kidney samples for Atlantic and pink salmon were compared at days 3, 6 and 9 post infection, and for chum salmon at day 6 only (total number of infected or control samples at all days = 57, 20, and 60 for Atlanti
Ns in an MX3000P (Agilent) as previously described [76], with the exception of running triplicate technical replicates. Genes of interest were selected based on relevance to the study system, presence in enriched functional categories, high significance or fold change, and relevance to multiple tissues. Reference candidates were selected based on other studies, unchanging expression in infected/co
Ments on the manuscript and statistical discussion. Thanks to B Cox for assistance in primer testing. Thanks to anonymous reviewers for comments on the manuscript. Author details 1 Centre for Biomedical Research, Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada. 2Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency
Control in the anterior kidney of all three species early in the infection. Boxplot displays median and interquartile range, and circles are outliers. *denotes p < 0.05; **denotes p < 0.001. Competing interests The authors declare that they have no competing interests. Authors' contributions BJGS contributed to experimental design, performed microarray, immunoassay and qPCR work and statistical an
Stocks. Aquaculture 2004, 232(1?):41?2. 31. Gjerde B, Odegard J, Thorland I: Estimates of genetic variation in the susceptibility of Atlantic salmon (Salmo salar) to the salmon louse Lepeophtheirus salmonis. Aquaculture 2011, 314(1?):66?2. 32. Jones CS, Lockyer AE, Verspoor E, Secombes CJ, Noble LR: Towards selective breeding of Atlantic salmon for sea louse resistance: approaches to identify trai
Stocks. Aquaculture 2004, 232(1?):41?2. 31. Gjerde B, Odegard J, Thorland I: Estimates of genetic variation in the susceptibility of Atlantic salmon (Salmo salar) to the salmon louse Lepeophtheirus salmonis. Aquaculture 2011, 314(1?):66?2. 32. Jones CS, Lockyer AE, Verspoor E, Secombes CJ, Noble LR: Towards selective breeding of Atlantic salmon for sea louse resistance: approaches to identify trai
Esponse of naive Atlantic, chinook and coho salmon to experimental infection with Lepeophtheirus salmonis (Copepoda: Caligidae). Dis Aquat Organ 1992, 14(3):179?93. Krasnov A, Skugor S, Todorcevic M, Glover KA, Nilsen F: Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis, cortisol implant, and their combination. BMC Genomics 2012, 13
Esponse of naive Atlantic, chinook and coho salmon to experimental infection with Lepeophtheirus salmonis (Copepoda: Caligidae). Dis Aquat Organ 1992, 14(3):179?93. Krasnov A, Skugor S, Todorcevic M, Glover KA, Nilsen F: Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis, cortisol implant, and their combination. BMC Genomics 2012, 13