Rtile range 2?) days;Of the remaining 3366 samples, there were 2718 (80.7 ) samples positive for ERV3 with PCR amplification Ct values ranging from 23?5 (median 36) cycles. Overall, ERV3 was not detected in 649 (19.2 ) samples. During the first 8-months of batching and screening conducted in the laboratory, the number of ERV3 negative samples ranged from 11 to 25 in each 92 extraction run with a m
Rtile range 2?) days;Of the remaining 3366 samples, there were 2718 (80.7 ) samples positive for ERV3 with PCR amplification Ct values ranging from 23?5 (median 36) cycles. Overall, ERV3 was not detected in 649 (19.2 ) samples. During the first 8-months of batching and screening conducted in the laboratory, the number of ERV3 negative samples ranged from 11 to 25 in each 92 extraction run with a m
T ambient temperature has limited or no impact on respiratory virus detection by PCR [14,20,21], although investigating further the effects of transporting samples for extended periods and at higher temperatures was highlighted in one study [20]. The observational research in childhood infectious diseases (ORChID) project is a longitudinal, communitybased, dynamic birth cohort study, which seeks t
Acer (ITS) region was performed using 10 pmoles of forward and reverse primers (ITS1 forward primer TCCGTAGGT GAACCTGCGG and ITS4-reverse primer TCCTCCGC TTA TTGATATGC [35], 25 L of Qiagen SYBR master mix (Qiagen, Australia) and 5 L of template in a total 50 L reaction mix. Cycling was performed using the following conditions: 95 for 15 min, 45 cycles of 95 for 30 sec, 50 for 30 sec and 72 for
Acer (ITS) region was performed using 10 pmoles of forward and reverse primers (ITS1 forward primer TCCGTAGGT GAACCTGCGG and ITS4-reverse primer TCCTCCGC TTA TTGATATGC [35], 25 L of Qiagen SYBR master mix (Qiagen, Australia) and 5 L of template in a total 50 L reaction mix. Cycling was performed using the following conditions: 95 for 15 min, 45 cycles of 95 for 30 sec, 50 for 30 sec and 72 for
Control testing were screened for respiratory viruses using previously optimized and described PCR and reverse transcriptase PCR assays. Virus testing assays included: rhinovirus (RV) [26], influenza viruses (A and B) [27], respiratory syncytial viruses (A and B) [28], parainfluenza viruses (1?) [29], human adenoviruses [22], humanTable 1 Oligonucleotide primers for equine herpes virus-1 (EHV 1) a
Standard deviation) time from collection until being frozen in the laboratory for samples with no observed mould was 2.9 (3.0) days. In comparison for low level mould it was 4.9 (3.6) days (crude mean difference compared with no mould; 95 confidence interval (CI) = 1.7; 1.4 ?2.1 days), for medium level mould it was 7.4 (4.9) days (3.9; 3.4 ?4.3), and for high level mould 11.4 (10.7) days (7.1; 6.
Standard deviation) time from collection until being frozen in the laboratory for samples with no observed mould was 2.9 (3.0) days. In comparison for low level mould it was 4.9 (3.6) days (crude mean difference compared with no mould; 95 confidence interval (CI) = 1.7; 1.4 ?2.1 days), for medium level mould it was 7.4 (4.9) days (3.9; 3.4 ?4.3), and for high level mould 11.4 (10.7) days (7.1; 6.