Al hold at 10 min at 95 ; followed by 45 cycles of 30 sec at 95 and 60 sec at 60 . The EHV1 component was performed as an extraction and inhibitor control as described previously [24], while ERV3 was used as a marker to evaluate the quality of nasal swab sample collection [25]. Briefly, the samples were considered to have failed the EHV1 component (ie. failed extraction or possessed PCR inhibitor