Coverslips have been incubated in blocking barrier (4�� SSC/0.1% Triton/5% bovine solution albumin) for Fifteen minutes at 70 degrees. Biotin-labeled #links# probes had been found using avidin-FITC (Vector Labs, Incorporated., Burlingame, California, U.S), together with FITC signals increased by further levels involving biotinylated anti-avidin (Vector Labs, Incorporated., Burlingame, Los angeles,