Five), MgCl2 (1), CaCl2 (3.A few), blood sugar (15), and also HEPES (12), ph 6.Four. The particular retinae were removed from your eyecups, segregated from retinal coloring epithelium, and incubated for twenty five minutes within 400 units/ml hyaluronidase (Worthington Biochemical Corp., Lakewood, New jersey, United states of america) with 70 degrees to eliminate residual vitreous. Retinae have be