2008; Langford-Smith avec al. 2010). The polyclonal anti-thrombin catch antibody and polyclonal anti-HCII recognition antibody (Affinity Biologicals) were chosen. DBS examples have been served by punching a new 3-mm-diameter sample through the Guthrie card employing a Wallac DBS Puncher (DELFIA) and were eluted with 200?��l Common Objective Solution Diluent (Serotec) with regard to 80?min with 70