2008; Langford-Smith ainsi que 's. The year of 2010). A new polyclonal anti-thrombin catch antibody along with polyclonal anti-HCII detection antibody (Love Biologicals) were utilized. DBS examples had been cooked by punching the 3-mm-diameter taste in the Guthrie minute card utilizing a Wallac DBS Puncher (DELFIA) and also ended up eluted together with 200?��l Basic Goal Solution Diluent (Serotec