Cells ended up analyzed #links# through FACS with regard to GFP-positivity (any), PI-exclusion (t), apoptotic nuclei (c) by developed for cleaved caspase Several as well as cleaved PARP (d). Tubulin was applied as a loading management. e, f ree p CSCs together with iCasp9 cellular material had been taken care of regarding 48?h using 500?��g/ml 5-FU and for 1?h with 10?nM dimerizer. Up coming, earl
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