1% (w/v) N-(1-naphthyl) ethylenediamine #links# using one particular a part of 1% (w/v) sulfanilamide inside 5% phosphoric acid solution. Following 30 minutes, absorbance ended up being measured with 540 nm using a UV/vis spectrophotometer (TU-1901, Purkinje Common, Beijing, Cina). The actual nitrite attention had been computed employing sea salt nitrite as being a regular. Lymphocyte growth exami