Immature maize embryos 8�C11?days following pollination ended up swamped utilizing a Bio-Rad PDS-1000/He Rifle (Bio-Rad, Hercules, Florida, United states of america) using a break stress involving 450?PSI. Postbombardment culture, choice, along with plant rejuvination had been done while earlier referred to (Gordon-Kamm et aussi . 2004). Cytological preparations along with FISH analysis FISH tests