Supernatant, which served as an extraction and inhibition control agent, before nucleic acid was extracted using the CAS1820 XtractorGene automated system (Qiagen-Australia) according to the manufacturer's instructions. The final volumes of specimen extracts were 150 L/specimen eluted in 96 well racks (Matrix, Thermo Scientific, Australia). For each run (96 extracts/run), extracts were tested usin