For marking involving neuromasts, are living zebrafish larvae ended up bathed in 3.Zero ��M AM1-43 inside embryo method for 30 to 45 mere seconds [66]. Soon after a number of clears within embryo channel. larvae had been anesthetized together with tricaine, set using 4% paraformaldehyde for two main a long time in 70 degrees, and also cryoprotected inside 30% sucrose right away as defined previous