Supernatant, which served as an extraction and inhibition control agent, before nucleic acid was extracted using the CAS1820 XtractorGene automated system (Qiagen-Australia) according to the manufacturer's instructions. The final volumes of specimen extracts were 150 L/specimen eluted in 96 well racks (Matrix, Thermo Scientific, Australia). For each run (96 extracts/run), extracts were tested usin
Supernatant, which served as an extraction and inhibition control agent, before nucleic acid was extracted using the CAS1820 XtractorGene automated system (Qiagen-Australia) according to the manufacturer's instructions. The final volumes of specimen extracts were 150 L/specimen eluted in 96 well racks (Matrix, Thermo Scientific, Australia). For each run (96 extracts/run), extracts were tested usin
Al hold at 10 min at 95 ; followed by 45 cycles of 30 sec at 95 and 60 sec at 60 . The EHV1 component was performed as an extraction and inhibitor control as described previously [24], while ERV3 was used as a marker to evaluate the quality of nasal swab sample collection [25]. Briefly, the samples were considered to have failed the EHV1 component (ie. failed extraction or possessed PCR inhibitor
Al hold at 10 min at 95 ; followed by 45 cycles of 30 sec at 95 and 60 sec at 60 . The EHV1 component was performed as an extraction and inhibitor control as described previously [24], while ERV3 was used as a marker to evaluate the quality of nasal swab sample collection [25]. Briefly, the samples were considered to have failed the EHV1 component (ie. failed extraction or possessed PCR inhibitor
Ater Brisbane metropolitan area and none were from rural communities. One-hundred and twentyfive recruits remained active study participants up until the date of this analysis. Of the 27 withdrawals, four had moved out of the study area, two others were later deemed ineligible, ten withdrew for personal reasons and eleven were ineligible because they could not fulfill sampling requirements. For th
Ples that failed EHV1 criteria or were not inspected for mould growth were excluded from the analysis (Figure 1).Data analysisERV3-TM Fam-TCTTCCCTCGAACCTGCACCATCAAGTCA-bhqSequences are listed 5` to 3`.The association between variables of interest and binary outcomes was investigated using mixed effects logistic regression models, with participants included as a random intercept to account for the
Ples that failed EHV1 criteria or were not inspected for mould growth were excluded from the analysis (Figure 1).Data analysisERV3-TM Fam-TCTTCCCTCGAACCTGCACCATCAAGTCA-bhqSequences are listed 5` to 3`.The association between variables of interest and binary outcomes was investigated using mixed effects logistic regression models, with participants included as a random intercept to account for the
Ater Brisbane metropolitan area and none were from rural communities. One-hundred and twentyfive recruits remained active study participants up until the date of this analysis. Of the 27 withdrawals, four had moved out of the study area, two others were later deemed ineligible, ten withdrew for personal reasons and eleven were ineligible because they could not fulfill sampling requirements. For th