Al best hit to rsbN is found next to nearly all bldN orthologues in actinomycete genomes; but, strikingly, the RsbN-like proteins are much more divergent than their BldN target or most other families of orthologous proteins of actinobacteria (Fig 6). We speculate that this may imply differences in the signal responsiveness of different RsbN proteins, thereby contributing to the differences between
E difficult to be confident that reciprocal BLASTP hits between genomes are meaningful, particularly when the extent of amino acid identity falls well below levels that are typically seen for conserved housekeeping genes. For example, the general kind of anti-anti-sigma factor to which the BldG protein belongs is almost universally found among both Gram-positive firmicutes and actinobacteria; so t
Itive search for orthologues of the 147 S. coelicolor TTA-containing genes (or in some cases gene clusters). We identified 19 that were widespread and frequently TTA-containing in 13 other Streptomyces genomes (Table 2). In addition, a further 10 genes or gene clusters frequently had TTA codons, even though their S. coelicolor orthologues were TTA-free (asterisks in Table 2). As 27/29 of the TTA-c
Ehalf of the Federation of European Microbiological SocietiesG. Chandra K.F. ChaterTable 2. Streptomyces genes or gene clusters that frequently contain TTA codons* SCO number of gene *asterisk means TTA codons are absent from the S. coelicolor gene 0381*;0382*;0383 0683*;0684*;0685* Fraction of TTA-containing orthologues among 14 Streptomyces genomes (see note) 6/12; 5/9; 2/10 2/12; 4/13; 2/Func
Translation (Nguyen et al., 2003; Takano et al., 2003). Translational regulation is via a very rare UUA codon in the adpA mRNA, falling between the segments encoding the two domains of AdpA. UUA is the only one of the six leucine codons to comprise only A and U residues, so the corresponding TTA codon is comparatively rare in GC-rich genomes ?it occurs in only 147 chromosomal genes in S. coelicolo
Translation (Nguyen et al., 2003; Takano et al., 2003). Translational regulation is via a very rare UUA codon in the adpA mRNA, falling between the segments encoding the two domains of AdpA. UUA is the only one of the six leucine codons to comprise only A and U residues, so the corresponding TTA codon is comparatively rare in GC-rich genomes ?it occurs in only 147 chromosomal genes in S. coelicolo
Ehalf of the Federation of European Microbiological SocietiesG. Chandra K.F. ChaterTable 2. Streptomyces genes or gene clusters that frequently contain TTA codons* SCO number of gene *asterisk means TTA codons are absent from the S. coelicolor gene 0381*;0382*;0383 0683*;0684*;0685* Fraction of TTA-containing orthologues among 14 Streptomyces genomes (see note) 6/12; 5/9; 2/10 2/12; 4/13; 2/Func
Herefore be expected to overexpress rsbN, the resulting increase in anti-BldN activity might interfere with the expression of BldN-dependent genes and contribute significantly to the bald phenotype of bldD mutants. The most well-studied target of BldN is bldM, which encodes an orphan response regulator (Molle Buttner,FEMS Microbiol Rev 38 (2014) 345??2013 The Author. FEMS Microbiology Reviews pu