Supernatant, which served as an extraction and inhibition control agent, before nucleic acid was extracted using the CAS1820 XtractorGene automated system (Qiagen-Australia) according to the manufacturer's instructions. The final volumes of specimen extracts were 150 L/specimen eluted in 96 well racks (Matrix, Thermo Scientific, Australia). For each run (96 extracts/run), extracts were tested usin
Supernatant, which served as an extraction and inhibition control agent, before nucleic acid was extracted using the CAS1820 XtractorGene automated system (Qiagen-Australia) according to the manufacturer's instructions. The final volumes of specimen extracts were 150 L/specimen eluted in 96 well racks (Matrix, Thermo Scientific, Australia). For each run (96 extracts/run), extracts were tested usin
Ater Brisbane metropolitan area and none were from rural communities. One-hundred and twentyfive recruits remained active study participants up until the date of this analysis. Of the 27 withdrawals, four had moved out of the study area, two others were later deemed ineligible, ten withdrew for personal reasons and eleven were ineligible because they could not fulfill sampling requirements. For th
Ples that failed EHV1 criteria or were not inspected for mould growth were excluded from the analysis (Figure 1).Data analysisERV3-TM Fam-TCTTCCCTCGAACCTGCACCATCAAGTCA-bhqSequences are listed 5` to 3`.The association between variables of interest and binary outcomes was investigated using mixed effects logistic regression models, with participants included as a random intercept to account for the
Erved on 28.2 of swabs. In comparison mould detection rates were 31.0 in spring (crude odds ratio (OR); 95 CI = 1.08; 0.87 ?1.34), 15.8 in autumn (0.47; 0.37 ?0.59) and 13.7 in winter (0.40; 0.29 ?0.53). When considering samples that contained mould, thereTable 2 Results for respiratory viruses screening from 3366 parent collected nasal swab specimens between July 2011 and July 2012 and fulfi
Er or others), season specimen collected, and time from specimen collection to being frozen in the laboratory. Analyses were conducted using Stata statistical software v.11.0 (StataCorp, College Station, TX, USA).however 10.9 of swabs were received more than 7-days after their collection.Excluded samples:For EHV1 extraction and inhibition testing, 42 (0.81 ) DNA extracts failed the EHV1 criteria.
Er or others), season specimen collected, and time from specimen collection to being frozen in the laboratory. Analyses were conducted using Stata statistical software v.11.0 (StataCorp, College Station, TX, USA).however 10.9 of swabs were received more than 7-days after their collection.Excluded samples:For EHV1 extraction and inhibition testing, 42 (0.81 ) DNA extracts failed the EHV1 criteria.
Acer (ITS) region was performed using 10 pmoles of forward and reverse primers (ITS1 forward primer TCCGTAGGT GAACCTGCGG and ITS4-reverse primer TCCTCCGC TTA TTGATATGC [35], 25 L of Qiagen SYBR master mix (Qiagen, Australia) and 5 L of template in a total 50 L reaction mix. Cycling was performed using the following conditions: 95 for 15 min, 45 cycles of 95 for 30 sec, 50 for 30 sec and 72 for